Reproduction Abstracts

Introduction: Currently, one-third of amphibians species are threatened with extinction and assisted reproductive technologies (ART) play an important role in their genetic management. The first step in developing ART is to create protocols for obtaining gametes. The aim of this study was to develop a protocol for hormonal induction of spermiation in the Natterjack toad (Bufo calamita).Materials and methods: Males were divided into three groups ...